Lethal effect of a hyperthermic CO2 pneumoperitoneum on gastric cancer cells

Summary Objectives We investigated the lethal effect of a hyperthermic CO 2 pneumoperitoneum on gastric cancer cells. This could form the theoretical basis for further studies of the feasibility and safety of inflating hyperthermic CO 2 in the abdominal cavity of gastric cancer patients during laparoscopy. Methods An in vitro hyperthermic CO 2 pneumoperitoneum experimental model was built, where gastric cancer cell line SGC-7901 cells were grouped according to temperature. Cytotoxicity was detected using a cell counting kit; apoptosis was detected by Annexin V-FITC/PI flow cytometry and Hoechst 33342/PI fluorescent microscopy. Morphological alterations were observed by transmission electron microscopy. Invasion and migration were detected by a scratch test and by transwell migration, respectively. Results Cytotoxicity assays showed that a hyperthermic CO 2 pneumoperitoneum significantly inhibited the proliferation of SGC-7901 cells ( P P 2 were significantly reduced. Conclusions A hyperthermic CO 2 pneumoperitoneum had a lethal effect on gastric cancer SGC-7901cells by inhibiting their invasion and migration, and by inducing apoptosis.