The effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits

Objective To explore the effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits.Methods 18 rabbits were randomly divided into three groups: normal group,cholesterol diet group,DHAP treatment group.All rabbits were fed for 12 weeks.Serum TC,TG,LDL were measured in 0 and 12 weeks in three groups.At 12 week,we detected the aortic VCAM-1 positive percentage by immunohistochemistry assay and the expression of VCAM-1 protein by Western blot.Results DHAP can obviously decrease the level of TC TG and LDL(P0.05).The measurement of VCAM-1 positive percentage and the expression of VCAM-1 protein both in cholesterol diet group and DHAP treatment group were higher than normal group(P0.05),but pathologic changes in DHAP treatment group were lower than cholesterol group(P0.05).The measurement of VCAM-1 positive percentage and the expression of VCAM-1 protein were also lower than cholesterol diet group(P0.05).Conclusion VCAM-1 can be highly expressed in atherosclerosis.DHAP can not only decrease cholesterol but also inhibit expression of VCAM-1 which has the effect of anti-atherosclerosis.