p16, p63 AND ki67 Expression in Squamous Metaplasia, Cervical Glandular Hyperplasia, Cervical IntraepithelialNeoplasia and Cervical Cancer

Introduction: Persistent infection with human papilloma virus(HPV) has been the main cause of squamous metaplasia which in turn may lead to in situ changes and then cervical cancer. p16, p63 and ki67 immunohistochemistry (IHC) can be used as an ancillary method for the diagnosis of these lesions. Aim: To evaluate the expression of p16, p63 and ki67 in squamous metaplasia, cervical glandular hyperplasia, Cervical intraepithelial neoplasia (CIN), cervical cancer and correlate histological features with IHC patterns. Settings and Design: 100 histologically proven cases of above mentioned lesions were taken in a observational study conducted from august 2018 to 2020. Methods and Material: 100 formalin fixed paraffin embedded blocks from cervical tissue were subjected to IHC using p16, p63 and ki67. Statistical analysis used: Using the SPSS 10.0 statistical software program. P< 0.05 was considered statistically significant. Results: Positive rates of p16, p63 and ki67 expression in CIN III and cervical cancer were higher than those in CIN I and II but there was no significant difference between various grades of cervical squamous cell carcinoma. Conclusions: The p16, p63 and ki67 expression positivity increased with the increasing severity of cervical lesions. Thus, high expression of ki67, p63 indicates neoplastic progression and p16 identifies HPV 16 infection in the uterine cervix.