Influences of Acid Cellulase on Indigo Backstaining on Cotton

In this research, the influences of acid cellulase on indigo backstaining at cotton surface were investigated. It was observed that more amount of celluase protein bound on the cotton would result in higher backstaining, indicating the direct adsorption between cellulase and indigo. It was also found that smaller indigo particle could generate higher backstaining because of its bigger specific area. At last, nonionic surfactant AEO-9 and protease were added to investigate their ability to remove backstaining. It was concluded that both of them could reduce the backstaining efficiently.