Survival of desert algae Chlorella exposed to Mars-like near space environment

Abstract Desert was considered terrestrial analogues of Mars. In this study, dried cells of desert green algae Chlorella were exposed to Mars-like near-space environment using high-altitude scientific balloons. We found that while a majority of Chlorella cells survived, they exhibited considerable damage, such as low photosynthetic activity, reduced cell growth, increased cell mortality rate, and altered chloroplast and mitochondrial ultrastructure. Additionally, transcriptome analysis of near space-exposed Chlorella cells revealed 3292 differentially expressed genes compared to cells in the control ground group, including heat shock proteins, antioxidant enzymes, DNA repair systems, as well as proteins related to the PSII apparatus and ribosomes. These data shed light on the possible survival strategy of desert algae to near space environments. Our results indicated that Mars-like near space conditions represent an extreme environment for desert algae in terms of temperature, pressure, and radiations. The survival strategy of Chlorella in response to near space will help gain insights into the possibility of extremophile colonization on the surface of Mars and in similar extraterrestrial habitats.