Synthesis, characterization and spectroscopic studies of surfactant loaded antiulcer drug into Chitosan nanoparticles for interaction with bovine serum albumin

Abstract In the present study, Lansoprazole loaded Chitosan-Tween80 Nanoparticles (NLPZ) have been synthesized by ionotropic gelation method and characterized by FTIR, PXRD, DLS, SEM and UV-Vis. The intermolecular interactions of LPZ and NLPZ with BSA were investigated using fluorescence, UV–vis, circular dichroism, and molecular docking techniques. The spectroscopic techniques suggested effective binding of LPZ and NLPZ to BSA under physiological conditions. The S-V plot of BSA-LPZ system showed positive deviation highlighting the presence of both static and dynamic quenching. Hence, ground state complex model and sphere of action quenching model were used for the study of BSA-LPZ system. The K¬¬SV and Ka of BSA-LPZ system were higher as compared to BSA-NLPZ system. Thermodynamic parameters indicated that the binding reaction in both systems was exothermic and spontaneous, enthalpically driven and also hydrogen binding and Van der Waals forces played a major role in the interaction of BSA-LPZ and BSA-NLPZ systems.