Pecan (Carya illinoinensis) detection using a monoclonal antibody-based direct sandwich enzyme-linked immunosorbent assay

Abstract A commercially available (BioFront Technologies) monoclonal antibody-based direct sandwich enzyme-linked immunosorbent assay (ELISA) for pecan detection was evaluated. The assay can detect 0.5 ppm of pecan and reliably quantify >1.5 ppm of pecan with