The long-term prognosis of patients with heart failure and the gene polymorphism Gln27Glu of β2-adrenoreceptors

The aim: to determine the influence of Gln27Glu polymorphism of the β 2 -adrenergic receptor gene on the long-term prognosis of patients with heart failure. Material and methods. The study included 200 patients with heart failure. The clinical course of the disease was evaluated and a genetic study of Gln27Glu polymorphism of the β 2 -adrenergic receptor gene was done. The material for molecular-genetic research was peripheral blood leukocytes of patients. Isolation of genomic DNA from blood leukocytes for molecular genetic studies was carried out using a commercial "DNA-sorb-B" kit in accordance with the instruction for the kit. Primer sequences were used for the polymerase chain reaction. Results . An analysis of the distribution of genotypes of the polymorphic Gln27Glu locus of the β 2 -adrenoreceptor gene in patients with heart failure showed that the genotype Gln27Gln occurs in 33 % of cases; Glu27Glu – in 13 %; Gln27Glu – in 54 %. Carriers of the mutant allele (G) of the β2-adrenoreceptor gene have a high incidence of atrial fibrillation (35.6 % vs. 7.7 %) over 3 years of follow-up. Hospitalization (42.0 % versus 19.2 %) and the frequency of reaching the combined end point (hospitalization + death) (54.0 % vs. 30.8 %) are greater among patients who carry the mutated G allele compared to homozygous patients with the "wild" allele C, for 3 years of observation. Polymorphism of the Gln27Glu gene of the β 2 -adrenoreceptor does not significantly affect the three-year mortality of patients with heart failure. Conclusions . The carriers of the mutant allele (G) of β 2 -adrenergic receptors have a high incidence of atrial fibrillation, hospitalization and the achievement of a combined end point (hospitalization + death) for 3 years of observation, compared to homozygous patients with the "wild-type" allele C. The polymorphism of the gene Gln27Glu of the β 2 -adrenoceptors does not affect the three-year mortality of patients with heart failure