Oxidative injury induced by cadmium sulfide nanoparticles in A549 cells and rat lungs

AbstractBackground: Rod-shaped cadmium sulfide nanoparticles (CdS NPs) are becoming increasingly important in many industrial fields, but their potential hazards remain unknown.Objectives: This study aimed to explore the patterns and mechanisms of lung injury induced by CdS NPs.Methods: A549 cells and rats were exposed to two types of CdS NPs with a same diameter of 20–30 nm but different lengths, CdS1 (80–100 nm) and CdS2 (110–130 nm). The using doses were included 10 μg/ml and 20 μg/ml two types of CdS NPs for cellular experiments and five times dose of 20 mg/kg body weight for rats’ exposure. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue staining were used to detect the A549 cell mortality percentage. The levels of reactive oxygen species (ROS) were determined in A549 cell. The vigor of superoxide dismutase (SOD) and the contents of catalase (CAT) and malondialdehyde (MDA) were detected both in A549 cells and in rats’ serum and lung tissues. The cellular morphological changes were o...