Age-Related Factors in Experimental Modeling of Polymorbidity and Probiotic Therapy for Manifestations of Systemic Inflammatory Response Syndrome

The development and validation of a new experimental model that reflects the clinical characteristics of polymorbid pathology (including obesity, acute inflammation of the gastrointestinal tract, antibiotic-induced dysbiosis, and bone injury) with the general characteristics of systemic inflammatory response syndrome became the basis for testing of the efficiency of probiotics according to the age of experimental animals. The concentration of osteocalcin and matrix metalloproteinase-1 significantly increased in 7-month-old male Wistar stock rats under the influence of probiotics as compared to animals at the age of 9 months. A mixture of probiotic containing Lactobacillus acidophilus (LA-5) and Bifidobacterium animalis subsp. lactis (BB-12) strains at a concentration of 108 CFU per animal demonstrated its effectiveness in comparison with other used probiotics in relation to most of the studied parameters. Zinc-containing biomolecules (osteocalcin, bone fraction and total alkaline phosphatase, matrix metalloproteinase-1) are metabolic regulators modulated by probiotic strains.