BCL2/BCL-X(L) inhibition induces apoptosis, disrupts cellular calcium homeostasis, and prevents platelet activation.

Apoptosis in megakaryocytes results in the formation of platelets. The role of apoptotic pathways in platelet turnover and in the apoptotic-like changes seen following platelet activation is poorly understood. ABT-263 (Navitoclax), a specific inhibitor of anti-apoptotic BCL2 proteins, which is currently being evaluated in clinical trials for the treatment of leukemia and other malignancies, induces a dose-limiting thrombocytopenia. In this study, the relationship between BCL2/BCL-XL-inhibition, apoptosis and platelet activation was investigated. Exposure to ABT-263 induced apoptosis but repressed platelet activation by physiological agonists. Notably, ABT-263 induced an immediate calcium response in platelets and the depletion of intracellular calcium stores, indicating that upon BCL2/BCL-XL -inhibition platelet activation is abrogated due to a diminished calcium signaling. By comparing the effects of ABT-263 and its analog ABT-737 on platelets and leukemia cells from the same donor, we show for the first time that these BCL2/BCL-XL -inhibitors do not offer any selective toxicity, but induce apoptosis at similar concentrations in leukemia cells and platelets. However, reticulated platelets are less sensitive to apoptosis, supporting the hypothesis that treatment with ABT-263 induces a selective loss of older platelets and providing an explanation for the transient thrombocytopenia observed upon ABT-263 treatment.