Serum Serotonin And Other Biochemical Parameters In Conditions Of High- Calorie Diet In Rats.

Serotonin, also known as 5-hydroxytryptamine (5-HT), is involved in formation of satiety and maintenance of a normal energy homeostasis. The study was conducted on white nonlinear rats with initial weight 200-215 g. Experimental obesity was simulated by a long-term (15 weeks) feeding with a high calorific food. In order to study the mechanism of obesity development in the groups of experimental animals, 5 rats were being randomly selected every 3 weeks from baseline, to obtain biological material. Our analysis confirmed the implication of serotoninergic system in conditions of obesity alterations and found the augment level of tryptophan and serotonin in serum of obese rats. It was established the more intense synthesis of GLUT-4 in adipocytes. There were also observed multiple changes of insulin receptors content: decrease in adipocytes membrane fractions of obese rats and increase in adipocytes cytosol that may be a result of cell walls damage. It is known that insulin is a key regulator of intracellular translocation process of the GLUT-4 to plasma membrane, but also serotonin possesses such effect. Serotonin can influence the glucose distribution