Cystic degeneration in liver injury induced by CCl4 in SD rats

Objective: To explore the hepatic injury induced by CCl_(4)in SD rat.Method: 40 SD rats were allocated to male and female group,consisting of 20 animals/sex/group.SD rats were given at 2 mL·kg~(-1) of 10% CCl_4 through celiac injection per 3 day for 12 days.All rats were killed by anaesthesia of ethyl ether and bleeding through abdominal aorta at 12~(th) day.Liver tissue was fixed in 10% neutral formalin,embedded in paraffin,cut at a nominal thickness of 3 μm,stained with hematoxylin and eosin(HE),evaluated at by microscopic examination.Result: 19 cases with local necrosis,8 cases with fatty degeneration,9 cases with cystic degeneration and 2 cases with fibrosis were seen in group male.20 cases with local necrosis,9 cases with fatty cases degeneration,1 case with cystic degeneration and 1 case with fibrosis were seen in group female.The incidence of cystic degeneration in male group was found significantly higher than that in female group(P0.05),but the incidence of other lesions was no significant difference between male and female group.Conclusion: CCl_(4) induces local necrosis,fatty degeneration,fibrosis and cystic degeneration in SD rat.The incidences of local necrosis,fatty degeneration and fibrosis were no significantly difference between male and female rat,but the incidence of cystic degeneration in male rats was significant higher than that in female rats.