Effect of electroacupuncture at conception vessel and governor vessel on correlative signals of stress-injury-repair in cerebral ischemia reperfusion rats

Abstract Objective To explore the effect of electroacupuncture at conception vessel and governor vessel on correlative signals of inflammatory response stress-injury-repair in cerebral ischemia reperfusion rats. Methods A total of 216 rats were included in the experiment, of which 192 rats were randomly divided into four groups, including sham operation group (group A), model group (group B), governor vessel group (group C), and conception vessel and governor vessel group (group D), 48 rats in each group. The rats in each group were further divided into six sub-groups, including 12 h group, 24 h group, 48 h group, 72 h group, 96 h group and 144 h group. MCAO operation course was simulated in group A, and the common carotid artery, internal carotid artery and external carotid artery on the right side were exposed, without insertion of suture or treatment. After completion of modeling in group B, treatment was not conducted. After completion of modeling in group C, “Shuigōu ( GV 26)”, “Baihui ( GV 20)” and “Dazhuī ( GV 14)” of governor vessel were selected, and electroacupuncture device was connected with “GV 14” and “GV 20”. After completion of modeling in group D, “GV 26”, “GV 20” and “GV 14” of governor vessel, and “Qihai ( CV 6)”, “Guānyuan ( CV 4)” and “Chengjiāng ( CV 24)” of conception vessel were selected; electroacupuncture device was connected with “GV 14” and “GV 20”, “CV 4” and “CV 6”. Neurological severity score was conducted. Enzyme linked immunosorbent assay (ELISA) was applied to determine the concentration of peripheral serum adrenocorticotropic hormone (ACTH) of rats, and immunohistochemical method was adopted to detect the expression levels of tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1) in brain tissue of rats. The other 24 rats were also randomly divided into four groups mentioned above, 6 rats in each group, to detect cerebral ischemie volume. Results Compared with group B, the ischemia volume in brain tissue at 144 h after cerebral ischemia reperfusion decreased significantly in group C and group D ( P P P P P P Conclusion Electroacupuncture at conception vessel and governor vessel can protect brain through the intervention of the expression of correlative signals of inflammatory response stress-injury-repair of cerebral ischemia reperfusion.