Effect of anti-inflammatory/antioxidant agent on diabetic hairless mouse skin

Skin inflammation is associated with almost all cutaneous disorders. Diabetes mellitus also influences skin physiology producing dryness and accelerating ageing. The development of effective and safe topical anti-inflammatory/antioxidant agents may be beneficial in such cases. We hereby evaluated the effect of a novel multifunctional derivative (AK1) that combines structural moieties of tolfenamic acid and L-cysteine. Thirteen groups of male hairless mice were exposed to acute UVR (2,5 MED). 1% tolfenamic acid, L-cysteine ethyl ester (as reference agents), AK1 and excipient were topically applied daily on streptozotocin-induced diabetic (D) or non diabetic (ND) mice. The examined parameters were TEWL, skin hydration, redness, thickness, elasticity, oxidative stress and hydrophilic antioxidants. In ND mice, AK1 significantly reduced TEWL on the 4th and 11th day while hydration levels were higher on day 14 in the AK1 group compared to control groups (p Application of AK1 on the skin of both D and ND mice appears to exhibit an effective anti-inflammatory activity.