신경세포 분화와 산화스트레스에서 추출 조건에 따른 가물치 추출물의 효과

The present study provides in vitro evaluations of snakehead (Channa argus) extracts on neuronal cell differentiation and oxidative stress. This study was conducted to analyze the constituents of the extracts prepared using different extraction methods (room temperature extract, RE; green tea room temperature extract, GRE; enzyme room temperature extract, ERE; green tea enzyme room temperature extract, GERE) and to compare their biochemical properties. The contents of total amino acids in ERE and GERE were much higher than RE and GRE from Channa argus. ABTS radical scavenging activities in enzymatic hydrolysates (ERE and GERE) were higher than those of RE and GRE. In addition, RE and ERE caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells and the expressions of growth-associated protein (GAP)-43 and synapsin-1. Four types of Channa argus extract were added to PC12 cells damaged by hydrogen peroxide (H2O2), after which the survival rate of PC12 cells was measured. The survival rates of PC12 cells were 77.5±1.9%, 84.0±0.8%, 70.4±1.5%, and 81.1±0.9% at RE, GRE, ERE, and GERE, respectively. These cell survival rates were higher than that of the negative control group (70.0±2.0%) treated only with H2O2. Cellular toxicities induced by treatment with H2O2 were also significantly alleviated in response to treatment with RE, GRE, and GERE. Taken together, these results suggest that Channa argus extracts are helpful as a functional material to decrease oxidative stress and neuronal damage.