P50 Up-regulated circPOLD1 promotes tumorigenesis in cervical cancer

Introduction/Background The role of circular RNAs (circRNAs) still need to be explored in cancers. We aimed to investigate differentially expressed circRNAs in onconegic progress of cervical cancer, and focus on one of them to verify its effects on the development in cervical cancer. Methodology High-throughput sequencing technique was performed to identify circRNAs expression profiles in cervical tissues of normal, High Squamous Intraepithelial Lesion(HSIL), and squamous-cell carcinoma (SCC)(each n=6). The results were validated by quantitative real-time PCR (qPCR) in 69 samples. The effect of circRNAs on proliferation, clonal formation and apoptosis were observed in cervical cancer cells by circRNA knockdown. RNA pulldown assay and RNA Binding Protein Immunoprecipitation Assay (RIP) was used to verify the combination between circRNA and its potential RNA binding protein, based on the bioinformatics prediction program. Results Totally 798 differentially expressed circRNAs with more than 2-fold change were identified(P Conclusion CircPOLD1 may play an oncogenic role on cervical cancer tumorigenesis by targeting YBX1. Disclosure Nothing to disclose.