Pathway Clustering of the Up-regulated Genes in the Stimulated Rice Seedlings that Germinating from the Seeds Implanted by the Low-Energy Nitrogen Ion

Abstract In order to understand the molecular mechanism of the ion-implantation biological effects, we analyzed genes expression profiles in rice seedlings from the seeds implanted with the nitrogen ion beam, using the Agilent Rice Oligo Microarray (4×44) Genome Array. Rice seeds were implanted by the nitrogen ion beam with 40 Kev energy in dose 6×10 17 N + /cm 2 . Total RNA, from the seedlings 96 h after the germination, was used to genechip analysis. The vigor index was measured at 10days after the germination. The results of measuring of the vigor index showed that smaller-dose implantation of the nitrogen ion beam (6×10 17 N + /cm 2 ) promoted the vigor index (P 17 N + /cm 2 ) damaged the rice seedlings because of the smaller vigor index than the control (P 2 and P value