An instant target for quantitative analysis

The present invention discloses an assay for a target real time quantitation method, comprising the steps of: (1) select the appropriate aptamers according to the target, and synthesizes a can and aptamer first portion of the sequence of the complementary to at least a chain of A, and and nucleic acid sequences complementary to a second portion of at least one suitable chain B; the chain a, the chain B are modified hydrogel monomer; (2) the modified monomer chain a, chain B polymerization, and contains the linker chain hybrid aptamer hydrogel-forming, while the signal amplification molecules, entrapped within the hydrogel structure; (3) adding the target in the hydrogel, the hydrogel collapsed target stimulation to release entrapped wherein the signal amplification molecule; solution phase placed substrate (4) SlipChip, molecules acting on the signal amplification solution phase, which catalyze a signal generating molecule may be read out SlipChip; (5) recording the detection result. The method is simple, low cost, fast response, no need for complex sample pre-treatment and so on.