A Semi-automated System of Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody.

African swine fever (ASF) is a highly contagious viral disease of domestic pigs and wild boars. For the disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (MB-LIPS) to detect ASF virus (ASFV) antibody. The MB-LIPS is based on magnetic beads modified with protein A/G and the recombinant fusion protein of ASFV p30 and luciferase, where p30 functioned as the recognition element and the luciferase as the signal component. Incubation and washing could be finished automatically on a machine with magnetic rods. Under the optimal conditions, the MB-LIPS showed 96.3% agreement to a commercial enzyme linked immunosorbent assay (ELISA) kit for detecting ASFV antibody in swine sera. Analyzing serial dilutions of a swine serum sample showed that the MP-LIPS assay was 4 times more sensitive than the ELISA kit. The whole run of the MB-LIPS could be completed within 30 min. With its high sensitivity and simple operation, the MB-LIPS platform has great potentials to be used for the detection of ASFV antibody and ASF control in small labs and farms.