A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization.

2015 
Abstract An electrochemical biosensor was developed for Hg 2+ determination based on DNA hybridization. In the presence of Hg 2+ , the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg 2+ –T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg 2+ . The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg 2+ in the range of 9.0 × 10 −11 –1.0 × 10 −9  M. The detection limit was 3.08 × 10 −11  M.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    8
    References
    9
    Citations
    NaN
    KQI
    []