A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization.
2015
Abstract An electrochemical biosensor was developed for Hg 2+ determination based on DNA hybridization. In the presence of Hg 2+ , the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg 2+ –T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg 2+ . The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg 2+ in the range of 9.0 × 10 −11 –1.0 × 10 −9 M. The detection limit was 3.08 × 10 −11 M.
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