Functional and Sustainable 3D Human Neural Network Models from Pluripotent Stem Cells

Three-dimensional (3D) in vitro cell and tissue culture models, particularly for the central nervous system, allow for the exploration of mechanisms of organ development, cellular interactions, and disease progression within defined environments. Here, we describe the development and characterization of human 3D tissue models that promote the differentiation and long-term survival of functional neural networks. This work builds upon previous work where primary rodent neurons were successfully grown in a similar 3D system.(1) The model was adapted to human induced pluripotent stem cells, allowing for a more direct exploration of the human condition. These tissue cultures show diverse cell populations, including neurons and astroglial cells, interacting in 3D and exhibit spontaneous neural activity confirmed through electrophysiological recordings and calcium imaging over at least nine months. This approach allows for the direct integration of pluripotent stem cells into the 3D construct, bypassing early ne...