Hydrogen peroxide preconditioning protects PC12 cells against MPP~+ toxicity through up-regulating 14-3-3 protein expression

Objective To investigate the protective effects of hydrogen peroxide preconditioning on the injury of PC12 cells induced by MPP+ and its mechanisms.Methods MTT assay,automatic biochemistry analyzer,DAPI stain and western blotting were utilized to determine the cell viability,LDH activity,apoptosis of PC12 cells and expression of 14-3-3 protein respectively.Results Hydrogen peroxide preconditioning could up-regulate 14-3-3 protein expression,increase cell viability (MPP+ group 52.46%±6.15%,HPP+MPP+group 83.78%±5.84%),inhibit LDH activity[MPP+ group(37.31±3.99)U/L,HPP+MPP+ group(12.49±2.26)U/L]and cell apoptosis(MPP+ group 48.72%±6.68%,HPP+MPP+ group 17.56%±5.21%).Conclusion Hydrogen peroxide preconditioning can protect PC12 cells against MPP+ toxicity through up-regulating the expression of 14-3-3 protein.