The use of ultasonication for preparation of nano-adjuvant rabies inactivated vaccine

The use of engineered nanomaterials (ENMs) that possess unique physicochemical properties in adjuvants enable researchers to achieve improved protection against infectious diseases. Recently, several studies were conducted on the use of nanoparticles as adjuvant to enhance the   immunogenicity of antigen and decrease the dose of immunologic adjuvants. In this work, we synthesized aluminum hydroxide nanoparticles using sonochemical technique nanoparticles, were characterized using X-ray diffraction, high resolution transmission electron microscope and zeta size potential. The result demonstrated that the size of aluminum hydroxide nanoparticles was 51 nm in diameter  that exhibited more potent antigen-specific antibody response than that of the micro-sized aluminum hydroxide gel particles (9 mm), so the prepared nano aluminum hydroxide was used as adjuvant for the inactivated rabies vaccine comparing with the traditional aluminum hydroxide gel and  the dose of  the prepared inactivated nana rabies vaccine was reduced, inducing high prolonged potent immune response in vaccinated dog.