Whole genome sequence of a blaNDM, blaKPC co-producing Klebsiella pneumoniae isolate KSH203 with capsular serotype K25 that belongs to ST11 from China

Abstract Objectives To characterize a high biofilm formation, hypermucoviscous, blaKPC and blaNDM co-producing Klebsiella pneumoniae strain, KSH203. Methods Antimicrobial susceptibilities, biofilm formation and hypermucoviscous phenotype were determined by the disk diffusion method, crystal violet staining and a positive string test, respectively. Whole-genome sequencing was performed using the Pacific Biosciences RS II Sequencer. High quality reads were de novo assembled using Celera Assembler v.8.0. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and the genome characteristic was analysed by bioinformatics methods. Results KSH203 was resistant to all antibiotics tested but only intermediary polymyxin B. This strain showed high biofilm formation ability, hypermucoviscous phenotype with serotype K25 belonging to the ST11 clone. KSH203 consists of a 5,464,059bp single chromosome and four plasmids including the pKSH203-NDM (53,144bp), pKSH203-KPC (159,467bp), pKSH203-CTX-M (156,910 bp) and pKSH203-qnrS (253,705bp). Forty-four antibiotic resistance genes and a large number virulence associated genes were identified in the KSH203 strain genome. Conclusions In this study, we mainly show the whole genome sequence of a high biofilm formation ability, hypermucoviscous K. pneumoniae isolate KSH203 with capsular serotype K25 that belongs to ST11 from a patient in China, which carried a large number of resistance genes and virulence-associated genes. Future studies are needed to vigilant this type of strain disseminate among environmental, animal and human.