Nitrogen deposition and increased precipitation interact to affect fine root production and biomass in a temperate forest: Implications for carbon cycling.

Abstract Fine roots connect belowground and aboveground systems and help regulate the carbon balance of terrestrial ecosystems by providing nutrients and water for plants. To evaluate the effects of atmospheric nitrogen (N) deposition and increased precipitation on fine root production and standing biomass in a temperate deciduous forest in central China, we conducted a 6-year experiment. From 2013 to 2018, we applied N (25 kg N ha−1 yr−1) and water (336 mm, 30% of the ambient annual precipitation) above the forest canopy, and we quantified fine root production and biomass in 2017 and 2018. At 0–10 cm soil depth, the statistical interaction between addition of N and water was not significant in terms of fine root production or biomass. At 0–10 cm soil depth, N addition significantly increased fine root production by 18.1%, but did not affect fine root biomass. Water addition significantly increased fine root production and biomass by 13.6 and 17.0%, respectively. Both N and water addition had significant direct positive effects on fine root production, and water addition had indirect positive effects on fine root biomass through decreasing soil NO3− concentration. At 10–30 cm soil depth, the statistical interaction between N addition and water addition was significant in terms of both fine root production and biomass, i.e., the positive effect of N addition was reduced by water addition, and vice versa. These findings indicate that fine roots and therefore belowground carbon storage may have complex responses to increases in atmospheric N deposition and changes in precipitation predicted for the future. The findings also suggest that results obtained from experiments that consider only one independent variable (e.g., N input or water input) and only one soil depth should be interpreted with caution.