Immunological rejection in brain of cloning goats received neural stem cell transplantation

Objective To investigate the immunological rejection in the brain of cloning goats received neural stem cell transplantation. Methods Eight cloning goats of CL series were chosen at random and divided into 2 groups. Neural stem cells and saline at the same dosages were transplanted into the fixed site by surgical intervention in the brain cortex of each group, respectively. The levels of IL-2 and IL-10 in the blood of each group were detected at different times (1 w before, and 0, 1 and 3 w,and 3 months after the cell transplantation) to reflect the systemic immune rejection of the goats after the transplatation. The CD3+ cells in the cell transplantation areas in each group were also detected by the method of immunohistochemistry to reflect the local immune rejection after the transplatation. Results The level of IL-2 was obviously higher and the level of IL-10 was obviously lower in the neural stem cell transplantation group than those in the control group 1 and 3 w, and 3 months after the cell transplantation (P＜0.05). The quantity of CD3+ cells in the neural stem cell transplantation group was much larger than that of control groups at the acute period (1 w after cell transplantation) and chronic period (3 months alter cell transplantation, P＜0.05). Conclusion Systemic and local immunological rejections at acute or chronic periods will appear at the brain of cloning goats with neural stem cells transplantation. Key words: Stem cell transplantation;  Immunological rejection;  Interleukins