The role of intercellular adhesion molecule-1,vascular cell adhesion molecule-1 and interleukin-8 in early acute respiratory distress syndrome

Objective:To observe changes in plasma interleukin8 (IL8),intercellular adhesion molecule1 (ICAM1),vascular cell adhesion molecule1(VCAM1) and their relationship to IL1β during hemorrhagic shock followed by a lowdose lipopolysaccharide (LPS) challenge.Methods:Twentytwo New Zealand rabbits were assigned to four groups.①In hemorrhagic shock group,shock was sustained by reducing cardiac output (CO) to 40% of baseline for 60 minutes,then resuscitation was performed with shed blood and maintained CO near baseline with Ringer solution for 4 hours.②In LPS group,animals received intravenously 1 001 25 μg/kg LPS.③In hemorrhagic shock plus LPS group,animals were intravenously infused the same dose of LPS,lasting for 4 hours after resuscitation for 60 minutes.④Normal control group.Results:In shock plus LPS group,VCAM1 levels significantly increased at 4 hours following LPS infusion compared to normal controls ( P 0 05).Also,plasma ICAM1 levels markedly elevated at 2 and 4 hours after LPS challenge(both P 0 05 ).The IL1β levels,which significantly increased after 60 minutes shock,were found in both shock plus LPS group and shock only group(both P 0 05).Plasma IL8 levels in all four groups did not significantly change at different intervals,except the values at 2 hours postLPS infusion (both P 0 05) in shock only and shock plus LPS group.Plasma IL1β levels in shock group returned to normal range after resuscitation,while it maintained high values at 2 and 4 hours after LPS infusion in shock plus LPS group(both P 0 05).Conclusions:These data suggest that hemorrhagic shock followed by a lowdose LPS challenge can lead to the elevation of plasma ICAM1 and VCAM1 levels,which might play important roles in increasing pulmonary vascular permeability.