Smartphone-based epifluorescence microscope for fresh tissue imaging

We developed a smartphone-based epifluorescence microscope for fresh tissue imaging. The smartphone microscope optics was optimally designed to achieve similar resolution (0.56 µm) and FOV (520 µm) as the bench 40x microscope, commonly used during the histopathologic analysis. Preliminary images obtained from an excised human pancreatic tissue stained with a rapid staining fluorescence dye (PARPi-FL) clearly visualized individual tumor cells.