Nonvalue of terminal aerobic subculture of unvented Roche Columbia broth blood culture bottles.

Abstract This study evaluated the need for a terminal aerobic blind subculture of unvented Roche Columbia broth. Only 2 of 2,871 bottles subcultured grew clinically significant organisms that were not also found in another blood culture. We conclude that in our tertiary care institution, blind subcultures of Roche Columbia broths are unwarranted.