Detecção molecular e análiseilogenética do gene H de amostras do vírus da cinomose canina em circulação no município de

Rosa G.N., Domingues H.G., Santos M.M.A.B., Felippe P.A.N., Spilki F.R. & Arns C.W. 2012. (Molecular detection and phylogenetic analysis of the gene H from cani- ne distemper virus isolates circulating at the municipality of Campinas, Sao Paulo.) Deteccao molecular e analiseilogenetica do gene H de amostras do virus da cinomose canina em circulacao no municipio de Campinas, Sao Paulo. Pesquisa Veterinaria Brasileira 32(1):72-77. Laboratorio de Microbiologia Molecular, Instituto de Ciencias da Saude, Uni- versidade Feevale, Rodovia RS-239 2755, Novo Hamburgo, RS 93352-000, Brazil. E-mail: fernandors@feevale.br Canine distemper virus (CDV), a Morbillivirus of the family Paramyxoviridae, is the etio- logical agent of neurological and systemic disease in dogs. The laboratory diagnosis of in- fection requires viral isolation or detection of genetic material of the virus in secretions or tissues of dogs with clinical suspicion of the disease. The genetic diversity among isolates of CDV can be assessed by sequencing and phylogenetic analysis of the gene that encodes the viral hemagglutinin (H gene), and there is currently a special interest in comparing the strains currently circulating in theield with the genogroup America-1, which comprises strains present in vaccines available in the market. In this study, the molecular detection of CDV gene H was performed from biological samples harvested ante-and post-mortem from 15 dogs with clinical signs suggestive of canine distemper in the metropolitan region of Campinas, Sao Paulo. Ten of the 15 dogs examined had at least one positive organ under molecular detection and the obtained amplicons were sequenced and further analyzed by molecular phylogenetic analysis. Similarly to what has already been reported on previous studies regarding the diversity of the gene H in other countries, the phylogenetic recons- truction obtained for the samples of cases of distemper from Campinas region showed they were grouped with the North American, European and Japanese newly described samples, a genetic group distinguished from classical samples of CDV, named America-1, which en- compasses the vaccine strains Snyder Hill, Onderstepoort and Lederle..