Complete Nucleotide Sequence of New Enterovirus Isolated from the Cerebrospinal Fluid of Meningoencephalitis Patients

An aseptic meningoencephalitis broke out in Yanbian prefecture of Jilin Province in June, 1996. We isolated 23 virus strains from the cerebrospinal fluid (CSF) and stool specimens of the patients. The isolated viruses could not be typed by neutralization test using the RIVM antiserum provided by WHO. RT PCR showed the viruses were enteroviruses. The serological test demonstrated the isolated virus was the pathogen of the aseptic meningoencephalitis. We determined the complete nucleotide sequences of two strains of the isolated viruses——Yanbian96 83csf and Yanbian96 85csf. The complete nucleotide sequences of Yanbian96 83csf and Yanbian96 85csf both were 7 456 nucleotide long including the 3′ poly(A) tail. The homogeneity of the nucleotide sequence between Yanbian96 83csf and Yanbian96 85csf was 99.8%. In comparison with the sequences of other enteroviruses in GeneBank through internet, the homogeneity of the complete nucleotide sequence was low of 77% and that of the sequence of structural region VP1 was low of 67%. The results demonstrate the isolated viruses belong to new genotype enterovirus.