Studies on the Pathogenesis of BIS (2-Chloroethyl) Sulfide (HD) Induced Vesication in Porcine Skin

Abstract : HD is a potent vesicant whose pathogenesis remains speculative. Previous work with the isolated perfused porcine skin flap (IPPSF) described the HD dose-response relationship. The present work includes a time-response study, probes the effects of alterations in basal perfusion parameters (glucose concentration, perfusate flow), and assesses the prostaglandin (PGE2, 6-keto PGF1a) profile of HD vesication. immunohistochemistry and immunoelectron microscopy created a map of the epidermal-dermal junction epitopes in HD-induced blisters. In the time response study, 10 mg/ml HD was applied to IPPSFs and terminated at 1,3,5 and 8 hrs. In the perfusion parameter study, IPPSFs were perfused at 0.5, 1.5 or 3.0 ml/min and 50, 120 or 400 mg/dl glucose concentration. Viability was assessed by glucose utilization, vascular resistance, LDH release, glucose lactate ratio, enzyme histochemistry, light and transmission electron microscopy. HD exposure was characterized by changes in nucleolar and mitochondrial structure, glucose utilization, lactate