Cytotoxic and anti proliferative activity of Hymenodictyon excelsum in Ehrlich Ascites Carcinoma Bearing Mice: In vitro and in vivo studies

Abstracts Objective : Anti proliferative Activity of Hymenodictyon excelsum (MEHE) Stem Bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice. Material and methods : Twenty four hours after intraperitoneal inoculation of tumor (EAC) cells in mice, MEHE was administered at 100 and 150 mg/kg body weight for nine consecutive days. On day 10 half of the mice were sacrificed and rest were kept alive for assessment of increase in life-span. The anti proliferative effect of HEME was assessed by evaluating tumor volume, viable and non-viable tumor cell count, tumor weight, haematological parameter and biochemical estimation. Results: MEHE showed significant decrease in (p<0.01) tumor volume, viable cell count, tumor weight and elevated the life span of EAC tumor bearing mice. Haematological profile such as RBC, haemoglobin, WBC and lymphocyte count reverted to normal level in MEHE treated mice. Conclusion : From the result it was showed that the extract has potent dose dependent anticancer activity and that is comparable to that of 5-fluorouracil.