Effect of culture medium and nutrient concentration on fatty acid content of Chaetoceros muelleri

Fatty acid content in microalgae can be modified by culture conditions. The purpose of this study was to determine the effect of nitrogen sources and their concentration in the polyunsaturated fatty acid (PUFAs) contents of cultured Chaetoceros muelleri. Chaetoceros muelleri was batch cultured at 19°C and a continuous light intensity of 150 mmol m -2 s -1 . Three different concentrations of culture medium (f, f/2 and f/4) were used. The experimental medium was