High resolution melting analysis (HRM) based on 16SrRNA as a tool for personal identification with the human oral microbiome

Abstract Personal identification plays an important role in the forensic practice. The conventional methods of personal identification including STR, SNP and InDel are focusing on the molecular characteristics of the human cell. Recently, researchers pay attention to human microbiome by the reason that the human microbiome is rich in amount and variable among people. The purpose of this study is to apply the human oral microbiome to forensic personal identification. We designed one general primer pair: 518 F&806R, which targeted the 16SrRNA. We conducted the high-resolution melting analysis (HRM) with the one primer pairs. The results indicated that oral microbiome from different people could be distinguished by using HRM based on 16SrRNA. This study showed that human oral microbiome could be a promising marker for the forensic personal identification application.