Regulatory effects of long non-coding RNA HIF1A-AS1 on ischemic myocardial reperfusion injury in rats

Objective To study the regulatory effects of long non-coding RNA HIF1A-AS1 on the myocardial ischemia reperfusion(I/R)injury and the related mechanism.Methods Myocardial I/R injury model was established with SD rats,and hypoxia reoxygenation(H/R)model was established with rat cardiac myocytes.si-HIF1A-AS1 was used to inhibit HIF1A-AS1 expression in the cardiac myoctyes.Then the mRNA expression of HIF1A-AS1 was detected by real-time PCR,the growth vitality of cardiac myocytes was investigated by MTT assay,the concentration of lactate dehydrogenase(LDH)in the culture media was detected by ELISA,and the autophagy-associated protein Beclin-1expression was observed by Western blotting analysis.Results HIF1A-AS1 expression was increased in cardiac muscle of rat I/R model and rat cardiac myocytes of H/R model.Inhibition of HIF1A-AS1 by siRNA protected the cardiomyocytes against H/R injuries,reversing the decreased growth vitality of cardiac myoctyes,increased LDH level in the culture media,and increased expression of autophagy-related protein Beclin-1induced by H/R stimulation.Conclusion Inhibition of long non-coding RNA HIF1A-AS1 might play aprotective role in I/R injury of cardiac myoctyes by inhibiting the excessive autophagy of cardiomyocytes.