Biochemical and immunological characterisation of Act d 10 a lipid transfer proteins from green kiwifruit

Methods Pulp and seeds of green kiwifruit were manually separated and homogenized. Act d 10 was purified from seed extracts by ion exchange chromatography and RPHPLC, and sequenced by an automatic amino acid sequencer. Act d 10 was evaluated in vivo by skin prick test (SPT) and in vitro by detecting IgE using streptavidin-conjugated CAP (Phadia, Sweden). Act d 10 was spotted on a customized ISAC microarray (PMD, Austria) for evaluating IgE prevalence in a larger cohort of allergic patients.