A New Rat Model for Diffuse Axonal Injury Using a Combination of Linear Acceleration and Angular Acceleration

Abstract Diffuse axonal injury (DAI) is a frequent form of traumatic brain injury, and is usually associated with long-lasting neurological impairments. A new experimental model was developed in the present study to induce DAI in rats by combining low linear and angular accelerations. In most clinical scenarios, DAI is caused by these two forms of acceleration in combination. In the injury-producing facility described here, the rat rotated instantly after it had sustained the impact that produced linear acceleration. Rats rotated rapidly 90° in the coronal plane at a peak angular acceleration of 137 ± 12 krad/sec2 with a duration of 33.7 ± 1.2 msec. The linear acceleration was applied to the rat's head by dropping a 450 g weight from a height of 0.9 m. Rats exposed to the combined accelerations took significantly longer to regain consciousness (11.9 ± 3.6 min) than control rats (p < 0.01) or rats subjected to purely angular or linear acceleration (p < 0.01). Although macroscopic damage was observed in all...