Application of the Gene-Ratio Method in the Diagnosis and Prognosis of a Variety of Human Diseases

A simple, highly accurate gene expression ratio-based methodology has been shown to be useful in the diagnosis and prognosis of cancer. This “Gene-Ratio Method” affords many advantages over similarly accurate mRNA-based assays that make it more suitable for clinical applications. It was determined that the gene ratio technique could be generally applied to other publicly available gene expression data representing a variety of normal and diseased human tissue types. This technique was able to distinguish in a statistically significant manner between normal and tumor tissues as well as among different tumor types and benign diseases using data from both cDNA and oligonucleotide microarray platforms.