Establishment and evaluation of rat model of endometrial injury

Objective To establish and evaluate a rat model of endometrial injury. Methods Totally 26 SD rats were selected, and left uterus of each rat was selected as the model group, the right uterus was selected as control group. The left uterine cavity was injected with 95% alcohol for 5 min, the right uterus was injected with normal saline. The uterus was removed after two estrogen cycles. The changes of endometrial morphology, the endometrial thickness, score the degree of endometrial injury were observed, and the expression of cytokeratin (CK18), vimentin, integrin b3 were detected by immunohistochemical method. Results Compared with control group, the uterine of model group was hard, uneven thickness, irregular shape, and uneven thickness of uterine wall. HE staining showed that the endometrial epithelial cells of model group arranged disorder, interstitial congestion, edema, decreased the number of glands. The endometrial thickness of model group was significantly thinner, and the degree of injury was significantly increased. The expression of CK18, vimentin and integrin b3 in the model group was significantly lower than that in control group, and the difference was statistically significant (P<0.05). Conclusion The animal model of rat endometrial injury can be established successfully and effectively by 95% alcohol. Key words: Animal model; Rat; Alcohol; Endometrial injury