Perfil fitoquímico e microbiológico do extrato das folhas de Achyrocline satureioides (Lam.) DC. (Macela)

Objective: Achyrocline satureioides (Lam.) DC. (Macela) is popularly known for its anti-inflammatory, antispasmodic and intestinal disorders. The present work aimed to analyze the microbiological activity and the phytochemical profile of the extract of the leaves of Macela characterizing their secondary metabolic groups and their constituents. Methods: Macela hydroethanolic extract was prepared. For microbiological analysis, the bacteria were incubated on blood agar and the fungi were cultured on Sabouraud Dextrose agar (ASD - Himedia®). The plates were incubated at 37 ° C and after 24 hours the digital caliper was used to check the diameter formed by the inhibition halo promoted by the extracts and controls. The phytochemical profile of the hydroethanolic extract of Macela was carried out through the spectrophotometer. The DDPH method was used to analyze the free radical scavenging capacity of the studied extract. Results: The microbiological analysis showed that there was no inactivation of the strains of the tested microorganisms. The phytochemical profile showed a high content of total phenolic compounds and flavonoids; and the DPPH analysis showed an interesting antioxidant capacity, but not similar to the synthetic BHT. Conclusion / Final considerations: The extract did not show antimicrobial activity against the analyzed bacteria and fungi, but it has a large amount of phenolic and flavonoid compounds and antioxidant activity.