Experimental study on the inhibitory effects of aminoguanidine on MFC stomach cancer in mice

OBJECTIVE:To investigate the inhibition effects of aminoguanidine (AG),a selective inhibitor of inducible nitric oxide synthase,on the proliferation of MFC stomach cancer cell line and therapeutic effects on the implanted stomach carcinoma. METHODS: MTT method was used to examine the effects of AG on the proliferation of MFC cell lines. Nitrite levels in MFC cells supernatants were tested by using the Greiss assay.Inhibition rates, intratumoral microvessel density (MVD), the positive rate of inducible nitric oxide synthase were evaluated respectively after the mice were sacrificed. RESULTS: 1)Over a concentration ranged from 0.1 to 100 mmol/L,AG had inhibited the growth of MFC cell significantly. Nitrite levels in MFC cells supernatants were evidently inhibited.2) The growth of implanted tumor of MFC stomach cancer was inhibited by 52.9%, 47.1% and 35.3% at groups treated with high, middle and low dosage of AG,respectively. The MVD was decreased significantly in treated group. The positive rate of iNOS in treated group was lower than that in control group. CONCLUSIONS: AG can significantly inhibit the proliferation of MFC in vitro and the growth of implanted stomach carcinoma mice in vivo,and also inhibit the expression of iNOS and decrease MVD in carcinoma biopsy.