Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation–Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin

A new type of thermosensitive dual-template epitope molecular imprinting polymer was prepared and coated on magnetic carbon nanotubes (MCNTs@D-EMIP) for simultaneous recognition of human serum albumin (HSA) and transferrin (Trf) via the strategies of dual-template epitope imprinting, metal chelation imprinting, and distillation–precipitation polymerization (DPP). C-terminal peptides of HSA and C-terminal peptides of Trf were selected as templates, zinc acrylate and N-isopropylacrylamide were used as functional monomers, and MCNTs@D-EMIP was prepared by the method of DPP. The two types of template epitopes were immobilized by metal chelation and six-membered ring formed with zinc acylate. MCNTs@D-EMIP was prepared in only 30 min, which was much shorter than other polymerization methods. The resultant MCNTs@D-EMIP showed excellent specific recognition ability toward HSA and Trf. The adsorption amounts of MCNTs@D-EMIP for HSA and Trf were 103.67 and 68.48 mg g–1 and the imprinting factors were 2.57 and 2.17,...