Quality Control of High-Throughput Library Construction Pipeline for KAPA HTP Library Using an Agilent 2200 TapeStation

Next Generation Sequencing requires the input of high molecular weight genomic DNA to construct quality libraries for whole genome bacterial sequencing. Large scale sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of the input DNA using high-throughput methods that are fast and cost effective. In this study, the Agilent 2200 TapeStation and Agilent 2100 Bioanalyzer Systems were used to assess a few critical quality control steps for library construction. With minimal manual intervention, the Agilent 2200 TapeStation System determined the quality of genomic DNA, fragmented DNA, and final libraries constructed from multiple types of foodborne pathogens. The Agilent 2200 TapeStation System provided a single platform that effectively evaluated the necessary quality control steps, which provided a distinct advantage to decrease the time needed for library construction and a common instrument methodology for quality control.