Expression of chemotactic factor macrophage inflammatory protein 2 following rat orthotopic liver transplantation

Objective To study the expression of chemotactic factor macrophage inflammatory protein 2(MIP 2)following ischemia reperfusion injury of liver after rat orthotopic live transplantation. Methods By using rat orthotopic live transplantation model, donor liver was preserved in lactic acid Ringer's solution for 4?h (4?℃). The rats were then divided into two groups, namely, 1 h after transplantation and 4 h after transplantation. False operation group served as control. The expression of MIP 2 mRNA in liver and protein expression of MIP 2 in serum, neutropil infiltration in liver and ALT of serum were detected. Results In the donor liver pretreated with cold preservation for 4?h, compared with control group, the expression of MIP 2 mRNA and serum albumin in the group of 1 h after transplantation was increased significantly ( P 0.01 ). The expression of MIP 2 mRNA and serum albumin in the group of 4 h was higher than that of 1 h group after transplantation ( P 0.01 ). The expression of MIP 2 mRNA and albumin were positive correlation to neutrophil infiltration and ALT of serum with the correlation coefficient being 0.88 , 0.92 , 0.90 , 0.93 respectively ( P 0.01 ). Conclusion MIP 2 might play an important upregulatory role in the ischemia reperfusion injury following rat orthotopic liver transplantation and become one of the most important factors in reperfusion injury of liver transplantation.