Development of an Indirect ELISA Using Recombinant Truncated Envelope Glycoprotein for Detection of Antibodies against Japanese Encephalitis Virus

[Objective] To explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein diary cow. [Method] A total of 36 Holstein cows at same parity and with similar milk yield were randomly divided into three groups,namely,control group,Chinese medicine group I and Chinese medicine group II. The cows in the Chinese medicine group I were fed basal diet containing 4% compound Chinese herb additive;those in the Chinese medicine group II were fed basal diet containing 3% compound Chinese herb additive;and those in the control group were fed common basal diet. All cows in the three groups were analyzed comparatively. [Result] Supplementing some compound Chinese herb additive into basal diet increased milk yield and anti-heat stress ability of Holstein cows,besides,it also decreased cow diseases like cow mastitis. The Chinese medicine group I generated the highest economic benefit of practical production. [Conclusion] Supplementing Chinese herb additive into basal diet is an effective way to relieve heat stress and increase milk yield.