Associations between-1304 T>G polymorphism in the promoter of mitogen-activated protein kinase kinase 4 gene and prostate cancer susceptibility

Objective To investigate the associations between-1304T ＞ G polymorphism in the promoter of mitogen-activated protein kinase kinase 4 (MKK4) gene and prostate cancer (PCa) susceptibility in the Han population in Jiangsu and Anhui.Methods A case control experiment was performed,consisting of 174 cases of newly diagnosed PCa and 252 cancer-free healthy controls.The peripheral blood genome DNA was extracted.The polymorphism of MKK4-1304 locus was analyzed by polymerase chain reaction-ligase detection reaction (PCR-LDR) technique.The associations between the susceptibility to PCa and different genotypes were compared.Results TT,TG,and GG genotypes were identified in the promoter of MKK4 gene (-1304T/G).Logistic regression analysis revealed there was no significant difference at MKK4-1304 site between TG,GG,and TG + GG genotypes and TT genotype ( OR =0.775,95％ CI =0.516-1.164; OR=0.650,95％ CI =0.216-1.956; OR =0.763,95％CI =0.514-1.135,respectively).However,in the stratification analysis,the decreased risk of PCa was associated with the-1304G variant genotypes (-1304TG/GG),particularly in the subgroups of subjects older than 70 years ( OR =0.575,95％ CI=0.348-0.951) and no-smokers (OR=0.477,95％ CI=0.252-0.906).Conclusion The MKK4-1304 polymorphism is not related directly to the susceptibility to PCa in the Han population in Jiangsu and Anhui. Key words: Prostate carcinoma;  Mitogen-activated protein kinase kinase 4;  Gene polymorphism