Microarrays revealed general-key genes responsive to implantation of low-energy N + ion beam in rice

Implantation with low-energy ion beam can induce various plant biological effects including stimulation, damage and mutation. But the adaptive mechanism altering gene expression in response to this stressor in plant is not fully understood. A highly thorough and comprehensive analysis to investigate the general-key genes responsive to implantation with low-energy ion beam in plant is important to understand the diverse mechanism. We used Agilent Rice Gene Expression Microarray to investigate the overlap differentially expressed genes between two independent implantation experiments underlying three ion fluences. Our microarray data showed that 26 up-regulated overlap genes and 6 down-regulated overlap genes were observed. The Gene Ontology terms analysis showed that genes with functions including kinase, transporter, acceptor, transcription regulation were involved in signaling and response biological processes in rice responding to the implantation with ion beam. Our data also implied that ABA signal transduction pathway is involved in response to ion-beam implantation in rice. The RiceNet co-expressed network analysis showed the direct and indirect co-expressed linkages among these up-regulated genes, which suggested some signal transduction pathways should be involved in this response and remained unclear. Our microarray data revealed the general and key genes responsive to the stress of implantation with low-energy ion beam during the rice germination. These data provide information on the candidate genes for further elucidation regarding the molecular mechanisms of the biological effects of ion-beam implantation and its application for improvement of corn.   Key words:  Microarray; key genes responsive to implantation of low-energy N+ ion beam; Rice