Expression Level and Clinical Significance of Gli1 in Patients with Myelodysplastic Syndrome

OBJECTIVE: To study the expression level and clinical significance of Gli1 gene in patients with myelodysplastic syndrome(MDS). METHODS: The positive rate of bone marrow CD34+ cells was detected by flow cytometry in 53 patients with MDS.Magnetic beads were used to separate CD34+ cells. The expression of Gli1 on CD34+ cells was detected by RT-qPCR, 25 patients with iron deficiency anemia were selected as controls. The relationship of Gli1 expression with clinical characteristics were analyzed. 方法: 对53例MDS患者采用流式细胞术检测骨髓CD34+ 阳性率，磁珠分选CD34+ 细胞，再用荧光实时定量 PCR法检测CD34+ 上Gli1基因的表达，以25例缺铁性贫血患者作对照，分析Gli1表达与MDS患者临床特征和预后的关系及其意义. 结果: MDS患者中Gli1表达水平（0.73±1.26）明显高于对照组（0.07±0.46)(P＜0.05)；Gli1表达水平与血小板计数、染色体分组和IPSS危险度分层均相关(P＜0.05）；Gli1高表达和低表达组患者中位生存时间[JP2]分别为7个月和20个月(P＜0.05)。多因素分析显示，Gli1和染色体分组是2个独立的不良因素（均P＜0.05）. 结论: Gli1在MDS中高表达，并与患者的临床特征及预后存在显著相关性，Gli1可能参与MDS的发生及发展.